Sample control developed and validated an alternative method for the analysis of hydrocyanic acid (HCN) in bone fruit

AuthorVladimir Stankov, mag. ing.
DateJune 29, 2022
TagSCIENCE
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Sample control developed and validated an alternative method for the analysis of hydrocyanic acid (HCN) in the bone fruit of apricot hives (Prunus armeniaca) are a good source of proteins, fibres and iron. However, they also possess a naturally occurring cyanogenic glycoside amigdaline, which is best known for being falsely promoted as an anti-cancer drug. It is found in many plants, but mostly in apricot or apricot seeds, bitter almonds, apples, peaches and plums.

Amigdaline, as it belongs to costing glycosides, may release, through enzymes, toxic hydrocyanic acid (HCN) in the organism, the maximum permitted concentration of which is under COMMISSION REGULATION (EC) No 1881/2006 of 19 December 2006 laying down maximum levels of certain contaminants in foodstuffs of 20 μg/kg for unprocessed whole, ground, crushed and chopped apricot nuclei placed on the market for the final consumer.

The Sample Control Laboratory developed a fast alternative method for the determination of amygdaline on UPLC-MS-MS through which the value for hydrocyanic acid (HCN) is easily obtained. The method is in our lab and validated. You can see the chromatogram of amigdaline in apricot trawls in Figure 1.

Kromatogram amingdalina

Figure 1 Amigdaline chromatogram in apricot stones on UPLC-MS/MS